Journal: Biology of Reproduction
Article Title: What has single-cell RNA-seq taught us about mammalian spermatogenesis?
doi: 10.1093/biolre/ioz088
Figure Lengend Snippet: Mouse spermatogenesis single-cell RNA-seq datasets.
Article Snippet: Cell Rep Total cell number 1204 53 510 (from 2 P5, 1 P10, 1 P15, 1 P20, 1 P25, 1 P30, 1 P35, 2 8–9 week mice) 34 633 1200–2500 per sample (from 1 P6, 1 P14, 1 P18, 1 P25, 1 P30, 1 8week mice) 19 659 (>50 mice) 31 163 (from 21 adult mice, 30 P6 mice) 62 600 (from 11 to 38 week WT, Mlh3 KO, Cul4a KO, Hormad1 KO, and Cnp mice) Not mentioned 71 175 (80 from P1.5, 48 from P3.5, and 47 from P5.5) Interstitial steroidogenic progenitor cells (6362 cells), SCs (1932 cells), fetal Leydig cells (521 cells), primordial germ cells (483 cells), and endothelial cells (180 cells) from E16.5 2500 (1250 each from 2 mice) 181 (71 from P3 WT, 53 from P7 WT, 57 from Rhox10 KO P3) First-Wave + + – + – + – – + + – – + Unselected steady-state spermtogenesis – + + + – + + – – – – + – Sorted Spermatogonia + – + + – + + + – – – – – Sorted Spermatocytes + – + + – + + – – – – – – Sorted Spermatids + – + + – + + – – – – – – Somatic cells – Sertoli cells, Leydig cells, Myoid cells, Endothelial cells, Marcrophages Sertoli cells, Leydig cells, Myoid cells, Endothelial cells, Macrophage, Inntate Lymphosid Sertoli cells, Endothelial, Hematopoietic cells, Smooth muscle – Sertoli cells, Peritubular cells (adult) Sertoli cells, Leydig cells – – Sertoli cells Endothelial cells, fetal Leydig cells, Sertoli cells Sertoli cells, Leydig cells – Validation methods Knockout, ChIP-seq RNA scope, ChIP-seq IHC, smFISH IHC – IHC, qRT-PCR Knockout, IHC Knockout, Transplantation, Bulk RNA-seq, IHC IHC, Function (RA inhibitior) Knockout, ChIP-qPCR, IHC, WB Knockout in Sertoli cells – Knockout, IHC scRNA-seq Chemistry/Method 3′ sequencing and full length sequence (SMART-seq2) 3′ sequencing and barcoding (10x Genomics) 3′ sequencing and barcoding (Oliginal Drop-seq) 3′ sequencing and barcoding (10x Genomics) 3′ sequencing and barcoding (SMART-seq2 and Microwell-seq) full length sequence (Fluidigm C1), 3′ sequencing and barcoding (10x Genomics) 3′ sequencing and barcoding (10x Genomics) 3′ sequencing and barcoding (10x Genomics) full length sequence (Fluidigm C1) full length sequence (Fluidigm C1) 3′ sequencing and barcoding (10x Genomics) 3′ sequencing and barcoding (10x Genomics) full length sequence (Fluidigm C1) Replication Replication unclear 1 replicate (P10, P15, P20, P25, P30, and P35) and 2 biological replicates (P5 and adult) 6 x samples from individual adult mice.
Techniques: Biomarker Discovery, Knock-Out, RNAscope, Transplantation Assay, Sequencing
